Fig. 5.

Comparison of NIR-AF and SW-AF in 12 to 13 months old WT and pigmented and albino Abca4^−/− mice. NIR-AF (red) and SW-AF (green) images of the different strains were taken under identical conditions. Yellow colour shows areas where NIR-AF and SW-AF superimpose. a) Overview of RPE and choroidal BF, NIR-AF and SW-AF. Note that there are no tissue structures visible in the BF image of the albino mouse, due to lack of melanin and lack of additional staining. AF Images are not post-processed to allow comparison of AF intensities. b) Magnified overlay of NIR-AF and SW-AF images of the RPE areas shown in (a). AF Images are post-processed to allow the localization of weak signals. An asterisk marks autofluorescent outer segments. N = 1–2 animals per group. BF: bright field; NIR: near-infrared; SW: short wavelength.