Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 14;48:264–276. doi: 10.1016/j.ebiom.2019.09.016

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 2 — Genetically inhibiting GPR4 suppressed the proliferation, migration of CRC cancer cells.

(a–b) GPR4 expression in mRNA (a), protein (b) level of different colorectal cancer cell lines. (c) CCK-8 cell proliferation assay of control or GPR4-silenced HCT116 and HT29 cells in pH 6.8 culture medium. (d) Colony formation assay of control or GPR4-silenced HCT116 and HT29 cells in pH 6.8 culture medium. Statistical of colony formation assay were presented in the right panel. (e) Cell invasion assay of control or GPR4-silenced HCT116 and HT29 cells in pH 6.8 culture medium. Statistical of cell invasion assay were presented in the right panel. (f) Wound healing assay of control or GPR4-silenced HCT116 and HT29 cells in pH 6.8 culture medium. Statistical of wound healing assay were presented in the right panel (g) 3D on-top growth assay of control or GPR4-silenced HCT116 and HT29 cells in pH 6.8 culture medium. Scale bars, 100 μm. Differences between the control and other groups in (c) were analysed by one-way ANOVA test (* P < .05; ** P < .01; *** P < .001). Differences between the groups in (d, e and f) were analysed by unpaired Student's t-test (* P < .05; ** P < .01; *** P < .001).