Fig. 4.

RNA extract from different numbers of worms. Results are presented as mean ± S.D. (n = 3). There is a linear relationship (y = 20.48x − 27.88, R² = 0.9976) between worm numbers and the yield of RNA extract. There is a correlation between the released RNA content after worm lysis measured by Qubit kit/fluorometer and RNA yield determined by Nano-drop 1000 Spectrophotometer after RNA extraction (Pearson correlation test: r = 0.9989, P < 0.0001).