. 2019 Sep 27;9(10):542. doi: 10.3390/biom9100542

Table 1.

Oligonucleotide primers used for gene expression analysis.

Group	Gene	Primer	Oligonucleotide Sequence	Product Size (bp)
Internal Control	Beta-actin	Forward	ACAACCTTCTTGCAGCTCCT	197
Internal Control	Beta-actin	Reverse	ACCCATACCCACCATCACAC	197
Ion-Exchanger	AE1	Forward	TCCCGCTACACTCAGGAGAT	118
	AE1	Reverse	CAGGGGCATAGCTCTCTTGT	118
	CAII	Forward	TGCTGGAATGTGTGACCTGG	101
	CAII	Reverse	CTCCCCCTCCGAATTGAAGT	101
	Na⁺/K⁺ ATPase	Forward	CAGCACTCGCTTTCCCTCG	189
	Na⁺/K⁺ ATPase	Reverse	GGCCAGGCAGCCATAGAATA	189
	H⁺/K⁺ ATPase	Forward	CCCCTGAGTACGTGAAGTTCG	168
	H⁺/K⁺ ATPase	Reverse	CCACAACCACAGCAATGAGTG	168
	CA IV	Forward	GTCTATGCCCTCAAGCACCA	114
	CA IV	Reverse	TTGGGCTCCTTGGCTTGAAT	114
	NBC1	Forward	GCTATCCCGGCTTTGCTAGT	153
	NBC1	Reverse	GAAGGAGCACACCACCATGA	153
	NHE3	Forward	ACTGCTTAATGACGCGGTGA	160
	NHE3	Reverse	GAAGGCGAAGATGACACCA	160
	Rhbg	Forward	TGTCCGCTACAACCACGAAA	96
	Rhbg	Reverse	TGGAAGCTTGGGTAGCGAAA	96
	Rhcg	Forward	CTCTTCGGCGTGTTCGTGC	200
	Rhcg	Reverse	CCTACAGCGCTGAACCCATA	200
	Pendrin	Forward	AGAACCAGGCCAAATCCAGG	83
	Pendrin	Reverse	CAAGTCTACGCATGGCCTCA	83
	H⁺-ATPase	Forward	TGCCTTCAGTTAGAGAGGCCGTGA	147
	H⁺-ATPase	Reverse	TGCCAAGAAGAGTCTGGGACAAGG	147
Glutamate and Glutamine Metabolism	XC-sys	Forward	GCATCGTCCTTTCAAGGTGC	150
	XC-sys	Reverse	AAGAGGTAATACGCCGGGAC	150
	EAAC1	Forward	AAACCACGGTGCTCGGTC	127
	EAAC1	Reverse	ACCGGCGTTTGTGAGGAATC
	Glutaminase	Forward	TGGGCATGATGTGTTGGTCT	199
	Glutaminase	Reverse	TACGCAGCAAACAGGAGGTT	199
	SNAT3	Forward	GGAACGGAGTGCTGAACGTG	83
	SNAT3	Reverse	CTGAAACCACCCCAGAGCAC	83
	PEPCK	Forward	TGCCATGGCTGCTATGTACC	89
	PEPCK	Reverse	TTTGGATGCTACGGCATGGT	89
TCA Cycle	Citrate synthase	Forward	TGCTACACAGAACCTCAGTTCAC	243
	Citrate synthase	Reverse	ATCTGACACGTCTTTGCCGA	243
	Aconitase	Forward	CCTGTACCTGACACTGCTCG	223
	Aconitase	Reverse	TGTAGTCAGAGGGGTCAGCA	223
	IDH	Forward	TGCAAAAATATCCCCCGCCT	144
	IDH	Reverse	GCCATCCTTTGGGGTGAAGA	144

Abbreviations: AE1: anion exchanger1, CAII: carbonic anhydrase2, CAIV: carbonic anhydrase4, NBC1: Na⁺-HCO₃⁻ co-transporter1, NHE3: Na⁺/H⁺ exchanger3, Rhbg: Rh family B glycoprotein, Rhcg: Rh family C glycoprotein, XC-sys: Cysteine/glutamate transporter, EAAC1: Excitatory amino acid transporter1, SNAT3: Na⁺-coupled neutral amino acid transporter3, PEPCK: Phosphoenolpyruvate carboxykinase, IDH: Isocitrate dehydrogenase.