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Zymographic analysis of gelatinase activity of secreted proteases. The gelatinolytic activity of supernatants from liquid cultures of V. alginolyticus (A,B) and V. parahaemolyticus (C,D) and was visualized by zymography, using gelatine as substrate. Gelatinolytic activity was measured either in presence of CaCl2 (A,C) or EDTA (B,D). Culture media were as follows: MB 0.2× (1); MB (2); MB+Gel (3); MB+AA (4); MB+YE (5); MB+Pept (6); MB+Glc (7); MB+Gly (8); MB+Fe (9); MB+ME (10).
