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Prediction and validation of target genes of plant-derived miRNAs in P. xylostella. (A–C). Putative binding modes of miR159a and Px-BJHSP1 (A), miR166a-3p and Px-BJHSP2 (B), and novel-7703-5p and Px-PPO2 (C) in CDS region. The mfe was indicated above the hybrid. (D–F). Relative expression levels of Px-BJHSP1, Px-BJHSP2 and Px-PPO2 at 24 h post-injection of corresponding miRNA agomir. Data were presented as mean ± SD (Student’s t test, * p < 0.05, ** p < 0.01, *** p < 0.001).
