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. 2019 Oct 19;9(10):629. doi: 10.3390/biom9100629

Figure 2.

Figure 2

Expression of HER2 and epidermal growth factor receptor (EGFR) in 5637 and AU565 cells. (a) A representative sequence chromatogram showing the presence of two transcripts encoding wild-type HER2 and the S310F mutant in 5637 cells. (b) Flow cytometry analysis of two cancer cell lines assessing their reactivity to cetuximab, pertuzumab, and trastuzumab. The cells were incubated with individual antibody using the recombinant scFv-human Cκ fusion protein. The amount of bound antibody was determined using Allophycocyanin (APC)-labeled anti-human Cκ antibody.