Table 1.

Steady-state kinetic parameters of substrates with YdjH^a

Substrate	kcat (s−1)	Km (mM)	kcat/Km (M−1 s−1)
1	0.11 ± 0.01	13.5 ± 2.5	7.8 ± 1.6
2	0.06 ± 0.01	44 ± 5	1.4 ± 0.2
3	0.025 ± 0.001	0.018 ± 0.001	1400 ± 100
4	0.0020 ± 0.0001	1.4 ± 0.3	1.4 ± 0.3
5	0.35 ± 0.01	14.5 ± 0.4	24.4 ± 0.8
6	0.055 ± 0.002	29.9 ± 1.2	1.8 ± 0.1
10	0.42 ± 0.03	2.2 ± 0.4	190 ± 40
11b	-	-	0.3 ± 0.01
12	16.2 ± 0.3	0.77 ± 0.03	21000 ± 1000
13	0.68 ± 0.01	6.3 ± 0.3	108 ± 5
14	0.22 ± 0.03	0.54 ± 0.15	410 ± 120
18	0.34 ± 0.03	4.7 ± 0.5	72 ± 7
19	1 ± 0.5	7.0 ± 4.5	123 ± 5e

^aMonitored using a coupled enzyme assay by following the oxidation of NADH at 30°C in 50 mM HEPES/K⁺ buffer with 100 mM KCl, pH 7.4.

^bSaturation of 11 could not be obtained, so the data was fit to a line with the slope of the line equal to k_cat/K_m.