Table 1. KI efficiency using different gRNA purification methods.
hEMX1 gRNA purification | tbx5a I2 gRNA purification | Ratio of fluorescence- positive F0 embryos |
---|---|---|
LiCl | LiCl | 18/119 (15.1%) |
LiCl | Ethanol | 8/103 (7.8%) |
Ethanol | LiCl | 0/94 (0.0%) |
Ethanol | Ethanol | 0/43 (0.0%) |