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. 2019 Oct 30;8:e48081. doi: 10.7554/eLife.48081

Table 1. KI efficiency using different gRNA purification methods.

hEMX1 gRNA purification tbx5a I2 gRNA purification Ratio of fluorescence-
positive F0 embryos
LiCl LiCl 18/119 (15.1%)
LiCl Ethanol 8/103 (7.8%)
Ethanol LiCl 0/94 (0.0%)
Ethanol Ethanol 0/43 (0.0%)