Video 6. Chromosome dynamics during GLMs.
Cell 1: ChrIV dynamics during a GLM. Cell is expressing Htb2:mCherry and TetR:GFP, and has TetO repeats inserted into ChrIV. The Chromosome IV sister chromatids clearly move into the daughter with the majority of the genome. Cell 2: ChrV dynamics during a GLM. Cell is expressing Htb2:mCherry and TetR:GFP, and has TetO repeats inserted into ChrV. The Chromosome V sister chromatids clearly move into the daughter with the majority of the genome. Cells 3 and 4: ChrXII dynamics during a GLM. Cell is expressing Htb2:mCherry and LacI:GFP, and has LacO repeats inserted into ChrXII. The Chromosome XII sister chromatids clearly remain behind despite the majority of the genome entering the daughter cell. For all movies and cells, sister chromatids separate simultaneous with anaphase initiation and correction of the GLM. Sister chromatids can then be identified in both mother and daughter cells. The blue arrow points to the mother cell during timepoints where it is experiencing the GLM. Timestamp is Hours:Min.