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. 2019 Nov 13;6:20. doi: 10.1186/s40694-019-0082-9

Correction to: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

Min Jin Kwon 1, Tabea Schütze 1, Sebastian Spohner 2, Stefan Haefner 2, Vera Meyer 1,
PMCID: PMC6852946  PMID: 31754436

Correction to: Fungal Biol Biotechnol (2019) 6:15 10.1186/s40694-019-0079-4

Following publication of the original article [1], the authors reported that Table 5 was missing in the published version, although it was originally submitted and reviewed along with the rest of the manuscript. The complete Table 5 is given in this erratum.

Table 5.

Practical guidance for the implementation of CRISPR technology in filamentous fungi based on data obtained for T. thermophilus in this study

Plasmid-based approach RNP-based approach
Preparation of nuclease Cloning of the nuclease into a plasmid prior transformation is mandatory. When constitutively expressed, risk of off-targets should be considered. When present on AMA-plasmid, the risk should be lower but still present Cloning of the nuclease into a plasmid allowing heterologous expression, e.g. in E. coli, is a prerequisite. Once established and purified, the nuclease can be aliquoted and stored prior to use. As the protein does not become expressed in the targeted fungus, the risk of off-targets should be very small
Preparation of guide RNA Plasmid-based, thus more stable during handling and storage Involves in vitro transcription, hence potentially sensitive to handling errors
Transformation procedure Easy Easy but requires preassembly of RNPs
Transformation rate Very high also with four targets

Very high for single and double targets

Low for three and four targets

Single-targeting efficiency of FnCpf1, AsCpf1, SpCas9 Locus-dependent Locus-dependent
Multiplex-targeting efficiency of FnCpf1 High (34 ± 6% in this study) Low (13 ± 2% in this study)
MTP-based down-scaling for FnCpf1 Possible with no loss in efficiency with respect to single and double targeting Possible with no loss in efficiency with respect to single targetinga

aDouble targeting was not tested

The original article has been corrected.

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Reference

  • 1.Kwon MJ, Schütze T, Spohner S, Haefner S, Meyer V. Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi. Fungal Biol Biotechnol. 2019;6:15. doi: 10.1186/s40694-019-0079-4. [DOI] [PMC free article] [PubMed] [Google Scholar]

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