Figure 6.

Mecp2^S80 phosphorylation is required for CCl₄-induced liver fibrosis. (A) Alanine transaminase (ALT) assessment at 24, 48, and 72 hours after CCl₄/olive oil injection in WT and Mecp2^S80A mice treated with acute CCl₄ or vehicle. (B) Histologic sections showing α-SMA staining from WT and Mecp2^S80A mice treated with acute CCl₄. Graph shows the percentage of the area positive for α-SMA staining (n = 6 per group). (C, D) Liver sections showing collagen staining (Picrosirius Red) and α-SMA from WT and Mecp2^S80A mice treated with chronic CCl₄ or olive oil vehicle. Associated graphs show the percentage of the area positive for Picrosirius Red or α-SMA staining. Photomicrographs are at original magnifications of ×40. (D) mRNA level of COL1A1, α-SMA, IL-6, MMP2, and TIMP1 quantified by qRT-PCR in WT and S80A mice treated with olive oil vehicle or chronic CCl₄. Error bars in relevant panels represent mean ± SEM. Statistical significance was determined by ANOVA or Student t test; ∗P<.05, ∗∗P<.01, ∗∗∗P<.001. Signif diff, Significantly different.