Figure 9.

Single-turnover reaction of fully reduced S5HH (30 μM) reacted with benzoate (2.35 mM) and O₂ (900 μM) at short and (inset) long times (concentrations after mixing) in standard buffer, 4 °C. The reaction was monitored using a stopped-flow spectrophotometer at 453 nm (blue) and 700 nm (red). Both time courses are an average of 5 replicates. The fits to summed exponential equations are shown as white dashed lines over the data. The kinetic parameters from the fits are shown in Table 2. Inset: Time course of the reaction monitored at 700 nm over 500 s showing the decay of the 700 nm intermediate.