Table 1:
Cell Line | rOATP1A1 | rOATP1A1 + rOATP1A4 | ||||
---|---|---|---|---|---|---|
Time after Zinc | 0 hr | 4 hr | 24 hr | 0 hr | 4 hr | 24 hr |
n | 4 | 6 | 4 | 4 | 6 | 4 |
Km | 6.31 ± 3.83 | 1.88 ± 0.98 | 1.38 ± 0.73 | 6.14 ± 4.45 | 3.76 ± 1.83 | 1.52 ± 0.34 |
Vmax | 24.75 ± 12.57 | 24.0 ± 12.0 | 153.3 ± 44.4* | 34.3 ± 20.5 | 38.5 ± 25.0 | 97.6 ± 45.9* |
a | 0.66 ± 0.99 | 0.68 ± 0.72 | 3.1 e-08 ± 8.5 e-09 | 1.00 ± 1.14 | 0.58 ± 0.53 | 0.19 ± 0.37 |
All cells were cultured for a total of 24 hours in the presence of 150 μM ZnSO4 for 4 or 24 hr or in its absence as indicated. Uptake of 35S-BSP was assayed at 24 hr as described in Methods.
p <0.03 as compared to cells at 0 or 4 hours after Zn addition.