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. 2019 Oct 8;6(5):ENEURO.0198-19.2019. doi: 10.1523/ENEURO.0198-19.2019

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Copyright © 2019 Papathanou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Double-FISH analyses of tdTom mRNA with Dat, Th, Vglut2, and Gad1 mRNAs identify multiple sites of tdTom/Vglut2 and tdTom/Gad1 double-positive cells in the brain of tdTom^DAT-Cre and tdTom^DAT-CreERT2 mice. Double-FISH analysis of (A) tdTom and Vglut2 mRNAs or (B) tdTom and Gad1 mRNAs in the midbrain of tdTom^DAT-Cre mice and (J, K) tdTom^DAT-CreERT2 mice. Higher magnification of tdTom/Dat, tdTom/Th, tdTom/Vglut2, and tdTom/Gad1 mRNA within (C–C’’’) the VTA and SN (D–D’’’) the arcuate nucleus (E–E’’’) and the PMH, (F’–F’’’) in the lateral septum (G–G’’’) the LHb, (H–H’’’) the basal amygdala, (I–I’’’) and the RMN of tdTom^DAT-Cre mice. L–T’’’, Similar expression analysis was performed in the tdTom^DAT-CreERT2 mice for (L–T) tdTom/Dat, (L’–T’) tdTom/Th, (L’’–T’’) tdTom/Vglut2, and (L’’’–T’’’) tdTom/Gad1 mRNA in the different regions (yellow arrows indicate examples of colocalization between tdTom and Dat, Th, Vglut2, or Gad1 mRNA; scale bars: 275 and 150 μm; insets: 50 μm). Arc, arcuate nucleus; LS, lateral septum, MHb, medial habenula; SN, substantia nigra; Arcuate nucleus; BLP, Basolateral amygdaloid nucleus posterior division; LHb, Lateral habenula; LS, Lateral septum, MHb, Medial habenula; PMH, Premammillary nucleus of the hypothalamus, RMN, Retromammillary nucleus; SN, Substantia nigra, SNc, Substantia nigra pars compacta; VTA, Ventral tegmental area.