Four-week-old male and female Smad158;Alb-Cre+ and littermate Cre- control mice were treated with a low iron diet for 4 weeks (n=5–8 per group). At 8 weeks of age, serum and livers were collected to determine (A) serum iron, (B) serum Tf sat, (C) liver iron, and (E) serum ALT. (D) Liver Hamp and (F) liver Col1a1 mRNA levels were measured by qRT-PCR. Transcript levels were normalized to Rpl19, and the average of Cre- control mice was set to 1. Data are presented as scatter plots with mean ± SEM or box plots with min to max whiskers. *P < 0.05, **P < 0.01, ***P < 0.001 relative to Cre- control mice on a low iron diet by Student’s t test.