Abstract
The FMS-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) gene mutation is present in ~20% of patients with de novo acute myeloid leukemia (AML). Patients with an FLT3-ITD mutation have a poor prognosis. However, the prognostic function of FLT3-ITD combined with other cytogenetic abnormalities are not clear. In the present study, a retrospective analysis of 103 newly diagnosed patients with AML was performed. The results revealed that the overall survival (OS) and recurrence-free survival (RFS) times were significantly longer in patients with an FLT3-ITD mutation combined with other favorable risk genes, compared with in those patients with a single FLT3-ITD mutation (P=0.0361 and P=0.0426). Sorafenib combined with chemotherapy significantly improved the overall response rate (ORR) when compared with mono-chemotherapy (P=0.039), but no significant differences were observed in the OS and RFS. In conclusion, favorable-risk cytogenetics may improve the clinical outcomes of patients with FLT3-ITD-mutated AML, but adverse-risk cytogenetics may not further worsen the prognosis. Sorafenib combined with chemotherapy may increase the ORR but would not result in a longer OS and RFS.
Keywords: acute myeloid leukemia, FMS-like tyrosine kinase 3-internal tandem duplication mutation, prognosis, favorable-risk cytogenetics, adverse-risk cytogenetics
Introduction
Acute myeloid leukemia (AML), a highly heterogeneous disease, is characterized by uncontrolled proliferative blasts (1). At present, the treatment of AML is challenging due to patient characteristics and intrinsic biological factors (2). FMS-like tyrosine kinase 3 (FLT3) gene mutations are present in ~30% of patients with de novo AML; internal tandem duplication (ITD) mutations in the juxtamembrane domain of FLT3 account for two-thirds of FLT3 mutations (3). Patients with an FLT3-ITD mutation have a poor prognosis, with a shorter remission duration and higher relapse rates compared with patients with FLT3-unmutated AML (3). The FLT3-ITD mutation usually coexists with other gene mutations or fusion genes; it has been reported that the prognosis of patients with FLT3-ITD and nucleophosmin (NPM1) double mutations was better compared with that in those with a FLT-ITD mono-mutation (4). Previously, research has indicated that the occurrence of FLT3-tyrosine kinase domain (TKD) and NPM1 double mutations were a highly favorable prognostic factor (5). However, the prognostic function of FLT3-ITD combined with other gene mutations or fusion genes is not clear.
Small molecule inhibitors that target FLT3, including sorafenib and midostaurin, have been revealed to be effective in the treatment of patients with FLT3-ITD-mutated AML; the multitargeted kinase inhibitor midostaurin combined with standard chemotherapy prolonged the overall survival (OS) and event-free survival times in patients with FLT3-mutated AML who were in remission after consolidation therapy and entered a maintenance phase compared with patients who received placebo plus chemotherapy (2). In addition, a novel irreversible FLT3 inhibitor, FF-10101, also demonstrated excellent efficacy against FLT3-mutated AML cells (6). However, the long-term efficacy of these novel FLT3 inhibitors, and whether they may be used as substitutes for hematopoietic stem cell transplantation (HSCT) for patients with FLT3-ITD mutations is currently unclear.
In the present study, a retrospective analysis was performed to examine the complete remission (CR), relapse and survival of newly diagnosed patients with FLT3-ITD-mutated AML with or without other gene mutations or fusion genes, and the effect of sorafenib was evaluated in patients with FLT3-ITD mutated AML.
Patients and methods
Patient population
Adult patients with AML (n=103; age range 18–87 years; mean age, 50 years; 62 men and 41 women) diagnosed between January 2013 and June 2018 at Huai'an No. 1 People's Hospital, Nanjing Medical University (Nanjing, China), including 23 patients harboring an FLT3-ITD mutation, who were treated with different treatment regimens, were retrospectively included in the present study. A total of 45 patients were revealed to be alive at the time of data collection. Patients with acute promyelocytic leukemia were excluded. The study was ethically approved by the Institutional Review Committee of Huai'an No. 1 People's Hospital, and written informed consent was obtained from all patients. The diagnosis of AML was established according to the criteria of the WHO classification (7), including clinical presentations and morphological, immunophenotype and recurrent cytogenetic abnormalities. All patients were analyzed based on their response to induction therapy, CR, relapse, OS and recurrence-free survival (RFS) rates.
Detection of FLT3-ITD and other associated genes
Multiple markers for the diagnosis of AML were identified, along with gene mutations, including AML1-ETO, CBFB/MYH11, MLL/AF6, MLL/AF9, MLL/ENL, dupMLL, DEK/CAN, EVI1, HOX11, BCR/ABL, FLT3-ITD, NPM1, CEBPA, C-kit and DNMT3A. Fusion genes were detected using multiple nested RT-PCR. Total RNA was extracted using Omega whole-blood RNA extraction kit (cat. no. R6616-02; Omega Bio-Tek, Inc., Norcross, GA, USA). Nested RT-PCR detection method and reaction system were used as previously reported (8,9). AML-associated mutated genes were detected using high-throughput sequencing technology. Total DNA was extracted using whole blood DNA extraction kit (cat. no. D3392-02; Omega Bio-Tek, Inc.), and genetic mutations were detected by Kindstar Global Medical Laboratory Center (http://www.kindstar.com.cn/kindstar/cn/platform.html) using Sanger sequencing, as previously described (9,10). Bone marrow samples were used for this, and each patient signed a bone marrow puncture written informed consent form.
Treatment regimens
All 103 patients received a ‘3+7’ chemotherapy regimen (60 mg/m2/day daunorubicin or 10 mg/m2/day idarubicin for 3 days, and 100 mg/m2/day cytarabine for 7 days with a 24-h intravenous pump maintenance). Out of the 23 patients harboring FLT3-ITD mutations, 7 were treated with sorafenib combined with a ‘3+7’ chemotherapy regimen for induction therapy. If the interim bone marrow (BM) examination, which was performed between days 14 and 21 of induction therapy, revealed residual leukemic blasts, a second course of induction chemotherapy comprising cytarabine (100 mg/m2/day) plus 2 mg/m2/day homoharringtonine was administered for 5 days. Patients who achieved CR usually received four to six courses of consolidation chemotherapy or allo-HSCT. Consolidation chemotherapy regimens included high-dose cytarabine (3 g/m2 twice a day on days 1 to 3) or intermediate-dose cytarabine (1 g/m2 for 4 days) plus daunorubicin (45 mg/m2/day for 3 days) or mitoxantrone (4 mg/m2/day for 3 days). The patients who achieved CR whose induction regimen contained sorafenib with chemotherapy received a continuous maintenance of sorafenib during the chemotherapy interval. Allo-HSCT was performed in patients who achieved CR at the discretion of the attending physician, usually following two courses of consolidation chemotherapy; however, decisions were often made on the basis of the patients' willingness, disease status, risk classification, human leukocyte antigen (HLA) matching and financial status.
Evaluation
CR was defined according to the standard criteria of <5% blasts in BM. Hematologic recovery was measured in terms of the absolute neutrophil (>1×109/l) and platelet (>100×109/l) counts in the peripheral blood. Clinical recurrence following CR was defined as the presence of ≥5% blasts in BM or re-appearance of leukemic blasts in the peripheral blood, or the presence of extramedullary disease. OS and RFS were calculated from the date of diagnosis. An event was defined as induction therapy failure, relapse following CR or mortality from any cause. Relapse was evaluated in patients who achieved CR using a cumulative incidence function with respect to competing risks.
Statistical analysis
Statistical analysis was performed using SPSS 24.0 (IBM Corp., Armonk, NY, USA) and data were presented as the means ± standard deviation/standard error of the mean. Pearson χ2 survival distributions were estimated using the Kaplan-Meier method and the differences were compared using the log-rank (Mantel-Cox) test. Categorical variables were compared using Pearson χ2 or Fisher Exact test. P<0.05 was considered to indicate a statistically significant difference.
Results
Patient characteristics
A total of 103 patients with AML were investigated during this period, 23 (22.3%) of which had an FLT3-ITD mutation. The median age of the entire cohort was 50 years old (age range, 18–87 years); 31 (30.1%) patients were aged ≥60 years. Patient characteristics and FLT3-ITD mutation statuses are summarized in Table I. Eleven out of 23 (47.83%) patients had both NPM1 and FLT3-ITD mutation. High leukocyte counts usually occurred in patients with FLT3-ITD mutations. In addition to NPM1, other gene mutations combined with the FLT3-ITD mutation were observed as follows: CEBPA (2/23, 8.69%), AML1-ETO (1/23, 4.35%), EVI1 (1/23, 4.35%), HOX11 (5/23, 21.74%) and dupMLL (2/23, 8.96%) mutations.
Table I.
Patient characteristics.
| Characteristics | Total (n=103) | FLT3-ITH(+) (n=23) | FLT3-ITH(−) (n=80) | P-value |
|---|---|---|---|---|
| Age (years), median (range) | 50 (18–87) | 51 (18–87) | 49.5 (18–76) | 0.138 |
| <60 years, no. (%) | 82 (79.61) | 15 (65.22) | 57 (71.25) | |
| ≥60 years, no. (%) | 31 (20.39) | 8 (34.78) | 23 (28.75) | 0.578 |
| Male, no. (%) | 62 (60.19) | 12 (52.17) | 50 (62.50) | |
| Female, no. (%) | 41 (39.81) | 11 (47.83) | 30 (37.50) | 0.372 |
| WBC (×109/l), median (range) | 15.29 (0.63–257.0) | 40.44 (1.55–257.0) | 9.71 (0.63–211.0) | <0.001a |
| HB (g/l), median (range) | 74 (40–134) | 65 (46–127) | 79.5 (40–134) | 0.842 |
| PLT (×109/l), median (range) | 44.0 (1–461) | 48 (4–219) | 41.5 (1–461) | 0.290 |
| Cytogenetics, no. (%) | ||||
| Abnormal karyotype | 44 (42.72) | 5 (21.74) | 39 (48.75) | |
| Normal karyotype | 59 (57.28) | 18 (78.26) | 41 (51.25) | 0.021a |
| Other abnormal genes no. (%) | ||||
| NPM1 | 18 (17.48) | 11 (47.83) | 7 (13.75) | 0.0001a |
| CEBPA | 18 (17.48) | 2 (8.69) | 16 (20.00) | 0.208 |
| AML1/ETO | 17 (16.50) | 1 (4.35) | 16 (20.00) | 0.074 |
| EVI1 | 9 (8.74) | 1 (4.35) | 8 (10.00) | 0.397 |
| HOX11 | 9 (8.74) | 5 (21.74) | 4 (5.00) | 0.012a |
| C-kit/D816V | 7 (6.79) | 0 (0) | 7 (8.75) | 0.141 |
| Dup MLL | 3 (2.91) | 2 (8.96) | 1 (1.25) | 0.061 |
| CBFB/MYH11 | 2 (1.94) | 0 (0) | 2 (2.50) | 0.281 |
| BCR/ABL | 2 (1.94) | 0 (0) | 2 (2.50) | 0.443 |
| DEK/CAN | 2 (1.94) | 0 (0) | 2 (2.50) | 0.443 |
| MLL/AF6 | 2 (1.94) | 0 (0) | 1 (1.25) | 0.590 |
| MLL/AF9 | 2 (1.94) | 0 (0) | 2 (2.50) | 0.443 |
| MLL/AF10 | 1 (0.97) | 0 (0) | 1 (1.25) | 0.590 |
| MLL/ELL | 2 (1.94) | 0 (0) | 2 (2.50) | 0.443 |
| NuP98/HOxC11 | 1 (0.97) | 0 (0) | 1 (1.25) | 0.338 |
PLT, platelet; WBC, white blood cell count; HB, hemoglobin; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation; NPM1, nucleophosmin 1.
Statistically significant P-value.
Treatment response
All 103 patients received a standard ‘3+7’ regimen of induction chemotherapy. There were no significant differences in the CR rate between the FLT3-ITD-positive and FLT3-ITD-negative groups (65.22 vs. 62.5%; P=0.812; Table II). The overall response rate (ORR) was 78.26% for the FLT3-ITD-positive group and 86.25% for the FLT3-ITD-negative group (P=0.351). The relapse rate was significantly higher in the FLT3-ITD-positive group compared with the FLT3-ITD-negative group (34.78 vs. 15.0%; P=0.034). One patient harboring an FLT3-ITD mutation received allo-HSCT, and still had CR status 6 months later. In the FLT3-ITD-negative group, 10 patients received autologous stem cell transplantation, 3 received allo-HSCT and 4 received micro-transplantation (11). Out of the 23 patients harboring FLT3-ITD mutations, 7 were treated with sorafenib combined with chemotherapy for induction therapy and 16 with mono-chemotherapy. The ORR was significantly higher in the sorafenib plus chemotherapy group compared with in the mono-chemotherapy group (P=0.039; Table III), but no significant differences in the CR and relapse rates were observed between these two groups.
Table II.
Treatment response between the FLT3-ITD positive group and FLT3-ITD negative group.
| Treatment | Total (n=103) | FLT3-ITH(+) (n=23) | FLT3-ITH(−) (n=80) | P-value |
|---|---|---|---|---|
| Therapy regimens | ||||
| Mono-chemotherapy, no. (%) | 96 (93.2) | 16 (69.57) | 80 (100.0) | |
| Sorafenib + chemotherapy, no. (%) | 7 (6.8) | 7 (30.43) | 0 (0) | |
| Treatment response | ||||
| NR, no. (%) | 16 (15.53) | 5 (21.74) | 11 (13.75) | 0.351 |
| PR, no. (%) | 22 (21.34) | 3 (13.04) | 19 (23.75) | 0.269 |
| CR, no. (%) | 65 (63.12) | 15 (65.22) | 50 (62.5) | 0.812 |
| ORR, no. (%) | 87 (84.46) | 18 (78.26) | 69 (86.25) | 0.351 |
| Relapse, no. (%) | 20 (19.42) | 8 (34.78) | 12 (15.00) | 0.034a |
| Post-remission treatment | ||||
| Autologous transplantation (%) | 10 (9.71) | 0 (0) | 10 (12.5) | |
| Allogeneic transplantation (%) | 4 (3.88) | 1 (4.35) | 3 (3.75) | |
| Micro transplantation (%) | 4 (3.88) | 0 (0) | 4 (5.00) |
NR, no response; PR, partial remission; CR, complete remission; ORR, overall response rate; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation.
Statistically significant P-value.
Table III.
Treatment of patients with FMS-like tyrosine kinase 3-internal tandem duplication mutation mutations.
| Treatment response (%) | Total (n=23) | Mono-chemotherapy (n=16) | Sorafenib plus chemotherapy (n=7) | P-value |
|---|---|---|---|---|
| NR, no. | 5 (21.74) | 5 (31.25) | 0 (0) | 0.039a |
| PR, no. | 3 (13.04) | 2 (12.50) | 1 (14.28) | 0.906 |
| CR, no. | 15 (65.22) | 9 (56.25) | 6 (85.71) | 0.172 |
| OR, no. | 18 (78.26) | 11 (68.75) | 7 (100.00) | 0.039a |
| Relapse, no. | 8 (34.78) | 4 (25.00) | 4 (57.14) | 0.136 |
NR, no response; PR, partial remission; CR, complete remission; OR, overall response.
Statistically significant P-value.
Sorafenib plus chemotherapy did not improve the OS and RFS of patients with FLT3-ITD-mutations who did not receive allo-HSCT
Patients with FLT3-ITD-mutated AML were associated with a poor prognosis due to a high relapse rate (12). In the present study, 103 patients with AML who completed at least one cycle of induction therapy were analyzed, and the OS and RFS rates in 23 patients harboring an FLT3-ITD mutation were revealed to be significantly lower compared with those in patients with FLT3-ITD-unmutated AML (P=0.0295 and P=0.0238, respectively; Fig. 1A and B). A number of FLT3 inhibitors have been revealed to specifically inhibit the proliferation of leukemia cells in preclinical models. However, first-generation FLT3 inhibitors in patients with relapsed/refractory FLT3-mutated AML revealed transient reductions in the number of blasts in the blood and bone marrow, but rarely CR (13,14). In the present study, 7 patients with an FLT3-ITD mutation received sorafenib plus chemotherapy, and their ORR was revealed to be higher compared with those of patients with FLT3-ITD-unmutated AML, but no significant differences in the OS and RFS were observed between the mono-chemotherapy and sorafenib plus chemotherapy groups (P=0.2735 and P=0.7302, respectively; Fig. 1C and D). These results indicate that sorafenib was not able to improve the long-term follow-up results of patients harboring an FLT3-ITD mutation, and should receive allo-HSCT as soon as possible.
Figure 1.
Comparison of OS and RFS in patients with FLT3-ITD-positive and -negative AML. (A) OS and (B) RFS in FLT3-ITD-positive and -negative groups. OS and RFS rates in 23 patients harboring an FLT3-ITD mutation were lower compared with those in FLT3-ITD unmutated patients (P=0.0295 and P=0.0238, respectively). (C) OS and (D) RFS in the mono-chemotherapy and sorafenib plus chemotherapy groups. There was no significant difference in OS and RFS between the mono-chemotherapy and sorafenib plus chemotherapy groups (P=0.2735 and P=0.7302, respectively). OS, overall survival; RFS, recurrence free survival; AML, acute myeloid leukemia; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation.
Outcome of patients harboring an FLT3-ITD mutation combined with other gene mutations or fusion genes
The prognosis and outcome in patients harboring an FLT3-ITD mutation combined with other mutations or fusion genes are unclear. In the present study, two groups of patients harboring an FLT3-ITD mutation combined with favorable-risk genes (FRGs; defined as the presence alone of the AML1-ETO, CBFB/MYH11 fusion gene or NPM1 mutation, and CEBPA double mutation) or adverse-risk genes (DRGs; defined as multiple gene mutations, specific genes associated with a poor prognosis for AML, and complex chromosomal karyotype abnormalities, for example FLT3-ITD, C-kit and DNMT3A mutations) were analyzed. A total of 75 patients with genetic abnormalities received standard regimens of chemotherapy. The survival of all patients with an FLT3-ITD mutation combined with FRGs were compared with those of patients with an FLT3-ITD mutation combined with DRGs; no significant differences in OS and RFS were observed between these two groups (P=0.3422 and P=0.6599; Fig. 2E and F). Among the 75 patients who received standard treatment, 35 had a single FRG, 7 had a single FLT3-ITD mutation, 11 had an FLT3-ITD mutation and FRGs, 17 had a single DRG, and 5 had a combination of FLT3-ITD mutation and DRGs (Table IV). Compared with patients with a single FLT3-ITD mutation, the OS and RFS were significantly longer in patients with an FLT3-ITD mutation together with FRGs (P=0.0361 and P=0.0426, respectively; Fig. 2A and B). No significant differences in OS and RFS were observed between patients with a single FLT3-ITD mutation and those with DRGs (P=0.8549 and P=0.7879, respectively; Fig. 2C and D).
Figure 2.
Comparison of OS and RFS between FRGs and DRGs in patients with FLT3-ITD-mutated AML. OS in (A) FRGs alone, (C) DRGs alone or (E) compared with each other, with an FLT3-ITD mutation. OS was significantly longer in patients with an FLT3-ITD mutation combined with FRGs (P=0.0361). There were no significant differences in OS between the patients with a single FLT3-ITD mutation and the patients with DRGs (P=0.8549). RFS in (B) FRGs or (D) DRGs combined with an FLT3-ITD mutation, or (F) the two compared with each other. RFS were significantly longer in patients with an FLT3-ITD mutation combined with FRGs (P=0.0426), but no significant differences in RFS were observed between the patients with a single FLT3-ITD mutation and those with DRGs (P=0.7879). OS, overall survival; RFS, recurrence free survival; FRGs, favorable-risk genes; DRGs, adverse-risk genes; AML, acute myeloid leukemia; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation.
Table IV.
FLT3-ITD mutation together with other genetic abnormalities in 75 patients with acute myeloid leukemia.
| FLT3-ITH status | Total (n=75) (%) | Favourable-risk genes (n=46) (%) | Adverse-risk genes (n=22) (%) | Neither (n=7) | P-value |
|---|---|---|---|---|---|
| FLT3-ITD (+) | 23 (30.67) | 11 (23.91) | 5 (22.73) | 7 (100.0%) | |
| FLT3-ITD (-a) | 52 (69.33) | 35 (76.09) | 17 (77.27) | 0 | 0.914 |
Neither, only FLT3-ITD mutation not together with other genes; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation.
FLT3-ITD mutations in normal karyotype (NK) and abnormal karyotype (AK)
The FLT3-ITD mutation was common in patients with NK. A number of patients with AK also had FLT3-ITD mutations. Out of the 23 patients with an FLT3-ITD mutation were analyzed, 6 were AK and 17 NK; no significant differences in OS and RFS were observed between these two groups (Fig. 3A and B). Further analysis revealed that the 3-year OS and RFS were longer in patients with FLT3-ITD combined with NPM1 mutations compared with in patients with a single FLT3-ITD mutation, but there were no statistically significant differences (Fig. 3C and D). This result may be associated with the relatively small number of cases; further large sample studies are therefore required to confirm it.
Figure 3.
Comparison of OS and RFS in patients with different-karyotype FLT3-ITD-mutated AML. Comparison of (A) OS and (B) RFS between patients with FLT3-ITD-mutated AML with a normal and abnormal karyotype. There were no significant differences in the OS and RFS between these two groups. Comparison of (C) OS and (D) RFS between patients with a FLT3-ITD mono-mutation and FLT3-ITD together with NPM1-mutations. No significant differences in OS and RFS were observed between these two groups. OS, overall survival; RFS, recurrence free survival; AML, acute myeloid leukemia; FLT3-ITD, FMS-like tyrosine kinase 3-internal tandem duplication mutation; NPM1, nucleophosmin 1.
Discussion
AML is a heterogeneous disorder of hematopoietic progenitor cells, characterized by blocked differentiation and uncontrolled proliferation (15). Sequencing of AML genomes has revealed that AML is genetically diverse and clonally heterogeneous with multiple mutations, with a majority of patients having >2 gene mutations and being clonally represented (16,17). The most frequently mutated genes in AML are NPM1 (25–35%), CEBPA (6–10%), RUNX1 (5–15%), FLT3-ITD (~20%), DNMT3A (18–22%), ASXL1 (5–17%), KIT (<5%) and TET2 (7–25%) (1). AML1-ETO, mutated NPM1 without FLT3-ITD, biallelic mutated CEBPA and CBFB-MYH11 were associated with a favorable prognosis (1), and EVI1, MLL-AF6, DEK-CAN and FLT3-ITD with adverse prognosis. FLT3-ITD is a distinct clinical entity associated with a poor prognosis (11,18,19). In accordance with the results of the present study, AML with FLT3-ITD tends to affect older patients with higher peripheral white blood cell counts, NK (20), shorter remission duration and higher relapse rates compared with patients with FLT3-unmutated AML.
The FLT3-ITD mutation has been revealed to usually coexist with other molecular genetics and cytogenetic alterations; however, the prognostic function of FLT3-ITD combined with other gene mutations or fusion genes is not clear. In the present retrospective study, 103 patients with AML were analyzed, and it was revealed that the OS and RFS rates in 23 patients harboring FLT3-ITD mutations were lower compared with those in patients without FLT3-ITD mutations, but no significant differences in the CR and OR rates were observed between these two groups. NPM1 and FLT3-ITD are two of the most common genetic abnormalities in AML. Patients with NPM1 mutations have a favorable prognosis, older patients with NPM1-positive and FLT3-ITD-negative genotype have a relatively favorable prognosis, and patients with an FLT3-ITD mutation have a poor prognosis (21). The prognostic effect of the NPM1 mutation in de novo AML may also be influenced by the relative abundance of the mutated allele (22). FLT3-ITD mutation rates were twice as frequent in NPM1 mutant AML, when compared with AML with wild-type NPM1 (22). The FLT3-ITD co-mutation has been revealed to diminish the favorable effect of the NPM1 mutation (23). Consistent with previous studies, the prognosis of patients with the FLT3-ITD and NPM1 co-mutation in the present study was improved following high intensity chemotherapy, when compared with the patients with a single FLT3-ITD mutation. However, no statistically significant differences were observed between these two groups, which may be due to the small patient number. FLT3-ITD also co-existed with other genetic abnormalities, including FRGs including CEBPA and AML1-ETO, and DRGs including N-RAS, NMT3A, EVI1, DupMLL and HOX11 (24,25). In the present study, 76 patients with AML with genetic abnormalities were analyzed and the survival of patients with an FLT3-ITD mutation combined with good prognosis genes were compared with those with an FLT3-ITD mutation combined with poor prognosis genes. The results revealed no significant differences in OS and RFS between these two groups, which may be due to the small sample size. Among the 76 patients who received standard treatment, the OS and RFS of patients with an FLT3-ITD mutation combined with FRGs were longer compared with those of patients with a single FLT3-ITD mutation. No significant differences in OS and RFS were observed between the patients with a single FLT3-ITD mutation and those with DRGs.
NK AML accounts for 50% of all AML and represents the third and largest broad cytogenetic category in AML (26). FLT3-ITD has been recognized as a marker of poor prognosis in patients with NK AML, but FLT3-ITD mutations are also commonly observed in patients with AK AML (27,28), and the prognostic function of the FLT3-ITD mutation in these patients was unclear. A total of 23 FLT3-ITD mutated patients receiving treatment were analyzed; 6 patients were AK and 17 NK, and no significant differences in the OS and RFS were observed between these two groups.
At present, tyrosine kinase inhibitors have been demonstrated to be effective in the treatment of FLT3-mutant AML. The multitargeted kinase inhibitor midostaurin, in combination with chemotherapy, has emerged as a novel standard treatment of patients with FLT3-ITD and TKD-positive AML. It was approved by the FDA for the treatment of AML in 2017 (2,29). In order to further improve the prognosis of patients with FLT3-mutated AML, selective and potent second-generation FLT3 inhibitors (including gilteritinib, crenolanib, FF-10101 and quizartinib) are being investigated in clinical trials. FF-10101 and quizartinib have each exhibited an excellent efficacy against AML cells and in patients with FLT3 mutations (6,29–32). High-dose daunorubicin or cladribine chemotherapy have also improved clinical outcomes following acquired drug resistance in FLT3-ITD-mutated AML (3,27). However, first-generation FLT3 inhibitors in patients with an FLT3 mutation have resulted in transient reductions in the number of blasts but rarely CR improvement (13,14). In the present study, 7 patients with an FLT3-ITD mutation received sorafenib plus chemotherapy and exhibited a slightly higher ORR, when compared with patients who were FLT3-ITD-negative; however, no statistically significant difference in OS and RFS was observed between the mono-chemotherapy and sorafenib plus chemotherapy groups. This may be due to the small number of patients that were treated with sorafenib, and future studies should expand the number of cases in order to confirm the conclusions of the present study.
In summary, the FLT3-ITD mutation has been identified as a marker of poor prognosis in AML, and the clinical outcomes of patients with FLT3-ITD-mutated AML combined with favorable cytogenetics was improved, when compared with those of patients with FLT3-ITD mono-mutated AML. OS and RFS have been prolonged due to the application of FLT3 inhibitors and adjustments in chemotherapy regimens. However, FLT3 inhibitors may lack a durable and deep response, which is mainly due to acquired drug resistance (3). Allo-HSCT should be performed as early as possible following CR in patients with FLT3-ITD-mutated AML, and maintenance therapy with FLT3 inhibitors may be recommended following allo-HSCT.
In conclusion, the present data demonstrated that favorable-risk cytogenetics may improve the clinical outcomes of patients with FLT3-ITD-mutated AML, but not those of patients with adverse-risk cytogenetic abnormalities. Sorafenib combined with chemotherapy may increase OR rates but could not improve OS and RFS.
Acknowledgements
Not applicable.
Glossary
Abbreviations
- AML
acute myeloid leukemia
- CR
complete remission
- OS
overall survival
- RFS
recurrence free survival
- NPM1
nucleophosmin 1
- FLT3-ITD
FMS-like tyrosine kinase 3-internal tandem duplication
- FLT3-TKD
FMS-like tyrosine kinase 3-tyrosine kinase domain
Funding
The present study was supported by the ‘333 Projects’ Foundation of Jiangsu Province (grant no. BRA2017243), the ‘533 Projects’ Foundation of Huai'an City (grant no. HAA201739), the Science and Technology Fund of Huai'an City (grant no. HAB201810) and the Science and Technology Fund of Jiangsu Commission of Health (grant no. H2018085).
Availability of data and materials
Not applicable.
Authors' contributions
ST, CW and LY conceptualized the original idea, designed the experiments and analyzed the data. ST wrote the paper. YC, YD, LS, YS, LL, BD and ZH collected the patient information, treated patients with the regimens and analyzed the data with statistical software. CW and LY revised the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The protocol was reviewed and ethically approved by the Institutional Review Committee of Huai'an No. 1 People's Hospital (Huai'an, China).
Patients consent for publication
A written informed consent form was obtained from each patient.
Competing interests
The authors declare that they have no competing interests.
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Data Availability Statement
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