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. Author manuscript; available in PMC: 2019 Nov 25.
Published in final edited form as: Vaccine. 2019 Jun 11;37(30):4184–4194. doi: 10.1016/j.vaccine.2019.04.071

Table 3.

Primers for construction and sequencing of recombinant KSHV antigen constructs.

Primer name Primer sequence (5′−3′)
Cloning Primers KSHV gpK8.1-Fc-His FWD AAAAAGCGGCCGCGCCACCATGAGTTCCACACAGATTCG
KSHV gpK8.1-Fc-His REV AAAAAACTAGTGTAAAGATGGGTCCGTATTTCTGC
KSHV gB-Fc-His FWD AAAAAGCGGCCGCGCCACCATGACTCCCAGGTCTAGATTGG
KSHV gB-Fc-His Rev AAAAAACTAGTTTTAATAAAATTTATGAATCCGGTAAC
KSHV gH-gL Gibson.FOR TTCTCGAGGATCCGCGGCCGCGCCACCATGGGGATCTTTGCGCTATTTG
KSHV gHgL Gibson.REV GCATGTGTGAGTTTTGTCACTAGTTGCGCGTCTTCTATACATGCC
Sequencing Primers FC-Hisseqprimer1 GTCGAGGTCTCGACGGTATCG
FC-Hisseqprimer2 CTCTATAGGCACACCCCTTTGG
FC-Hisseqprimer3 CGCGCGCCACCAGACATAATAG
FC-Hisseqprimer4 GCTTTAATAAGATCTCTAG
FC-Hisseqprimer5 TGCTGGGCACGGTGGGCATG
FC-Hisseqprimer6 GGGTCTTTTCTGCAGAAGCTTG
FC-Hisseqprimer7 CTGACCAAGAACCAGGTCAGC
FC-Hisseqprimer8 GACGCTCAAGTCAGAGGTGGC
FC-Hisseqprimer9 CTCCCCGTCGTGTAGATAACTAC
FC-Hisseqprimer1 0 CAATATTATTGAAGCATTTATC
FC-Hisseqprimer1 1 TGCGTAAGGAGAAAATACCGC