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. 2019 Nov 26;10(6):e01658-19. doi: 10.1128/mBio.01658-19

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Copyright © 2019 Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Increased biofilm formation in the mazF disruption strain is ica dependent. Quantitative CFU assay was used to measure the mass of biofilm. Biofilm formation in the mazF::Tn and mazF::Tn/ΔicaADBC strains were compared to the parental strain. (A) Biofilm formation of the mazF::Tn/ΔicaADBC strain was lower than that of strains lacking mazF expression alone. (B) PIA production in JE2 strains. The PIA production in the strain that lost mazF expression is much higher than that of other JE2 strains. (C) mazF::Tn/ΔicaADBC double loss-of-function strain reversed the animal mortality to wild-type strain levels. (D). Biofilm antibiotic tolerance of the double loss of function was lower than that of strains lacking mazF expression alone. All experiments were performed in triplicate. **, P < 0.01. Error bars represent 95% CI (95% confidence intervals).