Figure 7.

Oxygen consumption rate (OCR) in donor primary BECs following overnight exposure to MWCNTs, measured by Seahorse Extracellular Flux assay. OCR measurements at all time points were normalized to total well protein measured after the assay. Baseline OCR was significantly reduced in MWCNT-treated cells compared to controls. ATP-coupled respiration measured following the addition of oligomycin was not significantly altered by MWCNT treatment, however, total reserve capacity of mitochondria uncoupled by FCCP was reduced. No differences in non-mitochondrial respiration (following the addition of rotenone) were found. (N = 4, ANOVA, Tukey post-hoc, *p < 0.05).