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. Author manuscript; available in PMC: 2019 Nov 27.
Published in final edited form as: Epilepsia. 2019 May 14;60(6):1137–1149. doi: 10.1111/epi.15160

Figure 4. Wildtype α1 subunits were reduced in CeA in Gabrg2+/Q390X mice.

Figure 4.

A. Images of CeA from wildtype (wt) or heterozygous Gabrg2+/Q390X (het) mouse brain sections that were stained with rabbit anti-α1 (green) and mouse anti-β2/3 (red) subunit antibodies. The nuclei were stained with TO-PRO-3.

B, C. The α1 or β2/3 subunit fluorescence signals in somata or non-somata regions in the whole sampled field were measured subtracting the background value in the nuclei region from the total raw value of fluorescence (n = 28 neurons or sampled fields).

D-I. The total lysates of CeA from 2, 3 and 4 m old mice respectively (D-E) or isolated synaptosomes (Spm) by subcellular fractionation from 2–4 months old mice combined (F) were analyzed by western blot. The membranes were blotted with rabbit anti-γ2 subunit that only recognized the wt γ2 subunit (D, F) or mouse anti-α1 subunit (E) antibody. The normalized integrated density values (IDVs) of γ2 (E) or α1 (G) subunit protein were measured by Odyssey system (n = 6). The γ2 subunit (n = 6) (G, I) or α1 subunit (n = 6) (H) IDVs were normalized to internal control ATPase IDVs (LC) first and then normalized to wt subunit IDVs, which were arbitrarily taken as 1 (*p < 0.05, **p < 0.01, *** p<0.001 vs wt). Data were presented as mean as mean ± S.E.M.