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. 2017 Mar 9;18(6):570. doi: 10.1002/cbic.201700096

Corrigendum: Bio‐orthogonal Fluorescent Labelling of Biopolymers through Inverse‐Electron‐Demand Diels–Alder Reactions

Eszter Kozma, Orsolya Demeter, Péter Kele
PMCID: PMC6885926  PMID: 28303691

In Section 5, in the discussion of the work of Doll et al. (ref. [73]) we mistakenly used Ac4ManCCyc rather than Ac4GlcCCyc twice. The paragraph should read:

“Recently, Doll et al. used Ac4GlcCCyc to study the glycosylation states of specific intracellular proteins [O‐GlcNAc transferase (OGT), transcription factor Foxo1, tumor suppressor p53, serine/threonine kinas Akt1, actin‐binding protein vinculin and calcium/calmodulin‐dependent protein kinase CAMK4] in live cells. The target proteins were tagged with GFP and overexpressed in HEK293T cells incubated in Ac4GlcCCyc containing medium. FRET between GFP and TAMRA‐3 tagged glycan was detected by fluorescence lifetime imaging microscopy (FLIM). This approach revealed nuclear localization and regulation of Akt1 by O‐GlcNAcylation. This was the first demonstration of studying protein glycosylation states within living cells, stressing the power of bio‐orthogonal chemistry.”

The authors apologise for this error.


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