This article corrects:
An Actin Filament Population Defined by the Tropomyosin Tpm3.1 Regulates Glucose Uptake, authors: Anthony J. Kee, Lingyan Yang, Christine A. Lucas, Michael J. Greenberg, Nick Martel, Gary M. Leong, William E. Hughes, Gregory J. Cooney, David E. James, E. Michael Ostap, Weiping Han, Peter W. Gunning and Edna C. Hardeman
Traffic 2015; 16(7): 691–711.
The article Kee et al (2015), printed as part of the July 2015 issue of Traffic, contained a duplicated panel. The corrected figure is below. No conclusions are affected by this correction.
Figure 4.
Tpm3.1 regulates the pool size of filamentous actin in WAT. A) Representative images of filamentous actin (F‐actin) in WAT from WT, Tpm3.1 Tg and KO mice visualized with phalloidin. Insert in the upper left image is of an adipocyte at higher magnification showing F‐actin at the cell cortex (boxed area) and intense signal at the intersection between three adipocytes (arrow) due to blood vessels and stromal cells. Scale bars = 50 µm. B) Quantification of the phalloidin signal at the adipocyte cortex (500 adipocytes/section). Signal was measured in regions where neighboring adipocytes were in close association (see inset in A) to ensure only actin associated with the adipocyte membrane was measured. Data are expressed as fold changes from WT values (n = 5 mice/group). C) Representative western blots of total actin (C4 antibody) in WAT from WT (wt/wt), Tg (tg/tg) and KO (ko/ko) mice. D) Quantification of total actin blots corrected for total protein (Coomassie‐stained gel). Data are expressed as fold change from WT values (set at 1) from n = 4 mice/group. E) Representative images of filamentous actin (F‐actin) in WAT from WT and Tpm1.7 Tg mice visualized with phalloidin. Scale bars = 50 µm. F) Quantification of the phalloidin signal at the adipocyte cortex as in (B). Data are expressed as fold changes from WT values (n = 3/group). Data are mean ± SEM. Statistical differences are indicated by *p < 0.05 and + p < 0.01 (Kruskal–Wallis and Dunn multiple comparison tests).