Table 1. The list of clades and correspondent orthological genes for analyzed cereal species (barley, maize, rice and bread wheat) used for further experimental verification.
The column “Gene groups” indicates names of clades following the terminology proposed in Doroshkov & Bobrovskikh (2018) based on the data on evolutionary conservation in genes; the column “Sequence” contains information about primers designed for experimental analysis on bread wheat. The table also contains primers of the reference gene 18s rRNA.
| Gene group | Target ensembl loci | Sequence (F, forward; R, reverse) | Product size (bp) | Associated barley sequences | Associated maize sequences | Associated rice sequences |
|---|---|---|---|---|---|---|
| APX D | 5DL_433012_AA1398100 U_642188_AA2112960 5AS_392962_AA1266880 |
F-GGACATCACAGTGGCTCAAG R-GATACACCCTTAGGAGGGTCA |
231 | HV158476G00020 | None | OS12G07830 OS12G07820 |
| APX E | 4DL_342793_AA1122380 4AS_307333_AA1019630 4BL_320270_AA1033310 |
F-GGATATTGTTGCCCTCTCTGGT R-GGGTCAGTCAGCAGGGTTT |
183 | HV40855G00010 | ZM01G11990 ZM09G23350 |
OS03G17690 |
| APX F | U_641465_AA2095890 2BS_146384_AA0464000 2DS_179678_AA0608540 |
F-GGTCACACCCTGGGAAGAT R-CGGTCGGCAACTGAAGAAG |
139 | HV56215G00010 | ZM02G41460 | OS07G49400 |
| CAT A | 7AL_556567_AA1765730 7DL_602975_AA1973160 7BL_576925_AA1860110 |
F-ATGCAGGAGAACTGGAGAATAG R-TGCCTGTAGTTGAGTGGAATG |
107 | HV7888G00010 | ZM05G16210 | OS06G51150 |
| CAT B | 5AL_376544_AA1238210 4BL_320451_AA1039710 4DL_343177_AA1131150 |
F-CCACTACGACGGGCTCAT R-CGGGCTGCTTGAAGTTGTT |
170 | HV71788G00010 | ZM01G02380 | OS03G03910 |
| CAT C | U_640838_AA2076560 6DS_545065_AA1749890 6BS_513206_AA1634480 |
F-GAGGATCAAGAAGGAGAACGAC R-GCACAAAGTTTACACAGAACGG |
186 | HV1561906G00010 | ZM04G41390 | OS02G02400 |
| SOD A | 4AL_289135_AA0965570 7AS_571265_AA1846380 7DS_625535_AA2065460 |
F-GCCCAATCCCATCTGACAA R-TACTCAGCCCTTCGATTCTCA |
151 | HV56996G00010 | ZM09G06320 | OS06G02500 |
| SOD B | 7AS_570531_AA1837180 4AL_291448_AA0994630 |
F-TTTCAGGGAGGAGTTCATGC R-ACAGTATGCCAAGACACAAGG |
156 | HV2547265G00020 | ZM06G08270 ZM09G05010 |
OS06G05110 |
| SOD E | 2AS_114204_AA0365000 2BS_148548_AA0493640 2DS_179233_AA0605480 |
F-CGCCATGCTGGTGATCTT R-CAGCTCATGTCCACCCTTG |
164 | HV160480G00010 | ZM07G29600 ZM09G21130 ZM01G15520 |
OS03G22810 OS07G46990 |
| Reference: 18s rRNA | F-ATACGTGCAACAAACCC R-CTACCTCCCCGTGTCA |
288 |