TABLE 1.
Total sialic acids were collected from different cell lines via mild acid hydrolysis and analyzed using HPLCa
| Cell type | Species | Sialic acid content (%) |
|||
|---|---|---|---|---|---|
| Neu5Gc | Neu5Ac | 9-O-Ac | 7,9-O-Ac | ||
| A549 | Human | 2.21 | 96.40 | 1.39 | ND |
| HEK-293 | Human | 2.26 | 96.72 | 1.02 | ND |
| MDCK-NBL2 | Canine | 0.79 | 97.89 | 1.32 | ND |
| MDCK type I | Canine | 0.66 | 97.67 | 1.67 | ND |
| MDCK type II | Canine | 0.76 | 98.13 | 1.11 | ND |
| NLFK | Cat | 2.00 | 96.87 | 1.13 | ND |
| EqKc3 | Horse | 2.93 | 97.07 | ND | ND |
| NBL6 | Horse | 2.52 | 96.38 | 1.1 | ND |
| L cells | Mouse | 3.87 | 96.13 | ND | ND |
| A72 | Canine | 2.04 | 95.85 | 2.11 | ND |
| SiNEC | Swine* | 4.69 | 93.56 | 1.75 | ND |
| SiTEC | Swine* | 1.97 | 96.54 | 1.49 | ND |
a
Percentages of the total sialic acid collected are presented. Representative chromatograms for standard, HEK-293, A549, and MDCK-NBL2 are shown in Fig. S1 in the supplemental material. ND, not detected; *, primary swine nasal (SiNEC) and tracheal (SiTEC) epithelial cells (courtesy of Stacey Schultz-Cherry).