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. 2019 Dec 3;10(6):e02802-19. doi: 10.1128/mBio.02802-19

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Copyright © 2019 Dumigan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Innate cell recruitment in K. pneumoniae-infected porcine EVLP model. (A) CD11R3⁺ macrophages at baseline (0 h) and endpoint (4 h posttreatment) in BAL samples and tissue from caudal lobes mock infected (PBS) or infected with Kp52145 and strain 52145-Δwca_K2. (B) Granulocytes at baseline (0 h) and endpoint (4 h posttreatment) in BAL samples and tissue from caudal lobes mock infected (PBS) or infected with Kp52145 and strain 52145-Δwca_K2. (C) CD172⁺ dendritic cells at baseline (0 h) and endpoint (4 h posttreatment) in BAL samples and tissue from caudal lobes mock infected (PBS) or infected with Kp52145 and strain 52145-Δwca_K2. (D and E) Percentages of CD11R3⁺ macrophages positive for CD163 expression associated (GFP⁺) or not (GFP⁻) with Kp52145 and strain 52145-Δwca_K2 harboring plasmid pFPV25.1 Cm in BAL fluid (D) and tissue (E). In all panels, values are represented as means ± SEM from three independent experiments. **, P < 0.001; *, P < 0.05 (determined by unpaired t test).