TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description (purpose)a | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli NEB 5-alpha | Competent E. coli—DH5α derivative | NEB |
| Streptococcus gordonii DL1 | S. gordonii wild type | 75 |
| Streptococcus gordonii ΔltaS::JHMD1 | DL1 with in-frame deletion of SGO_1377 | This study |
| Streptococcus gordonii ΔltaSc | Complemented ΔltaS::JHMD1; SGO_1377 reinserted into the attB locus | This study |
| Streptococcus gordonii ΔsrtA | DL1 with a markerless deletion of srtA | 53 |
| Fusobacterium nucleatum ATCC 23726 | Wild type | ATCC |
| Actinomyces naeslundii ATCC 12104 | Wild type | ATCC |
| Porphyromonas gingivalis 4612 | Wild type | 76 |
| Plasmids | ||
| pJHMD1 |
S. gordonii ldh promoter—ermAM-pheS*(A316G) in pUC57; Ampr (markerless deletion system) |
53 |
| pFW5 | S. gordonii shuttle vector, Specr | 77 |
| pBPL11 | pFW5 derivative containing ltaS ORF and its promoter (complementation) | This study |
a
Ampr, ampicillin resistance; ORF, open reading frame; Specr, spectinomycin resistance.