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. 2019 Sep 26;213(4):1415–1430. doi: 10.1534/genetics.119.302415

Figure 1.

Figure 1

Multiple stressors during adolescence induce neurite outgrowth of the DVB neuron in adult male C. elegans. (A) Illustration of male C. elegans with DVB neuron and neurites depicted. (B) Timeline of postembryonic development of male C. elegans with inset of L4 to adult period. Boxes below timeline indicate timing of different stressors labeled for each panel; gray indicates control/fed, magenta indicates starvation/fasting, teal indicates heat stress, and green indicates UV exposure. Asterisk indicates time of confocal imaging and DVB neurite quantification. (C) Confocal z-stack maximum projections of DVB neuron in control and mid-L4 stressed males at day 1 of adulthood (arrowheads indicate DVB neurites, white asterisk indicates soma of PVT neuron). Bar, 10 μm. Quantification of (D) total neurite length and (E) neurite junctions for DVB in control and mid-L4 stressed males at day 1. Each dot represents one worm; colored bar indicates the median, boxes indicate quartiles. Comparison using one-way ANOVA and post hoc Tukey’s test, P values shown above plots with bold showing significance (P < 0.05). True for all subsequent figures.