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. 2019 Sep 26;213(4):1415–1430. doi: 10.1534/genetics.119.302415

Figure 4.

Figure 4

Adolescent stress differentially affects adult spicule protraction behavior and number of DVB presynaptic active zone sites. (A) Cartoon of C. elegans male tail with DVB and spicules depicted in retracted and protracted positions. (B) Logic diagram of the male C. elegans spicule protraction circuit involving DVB GABAergic and SPC cholinergic signaling, showing location of action of the acetylcholinesterase inhibitor aldicarb. (C) Time to protraction of spicules in control and mid-L4 stressed males on aldicarb plates at day 1 of adulthood. (D) Confocal z-stack maximum projections of DVB neuron and DVB presynaptic puncta (lim-6int4::cla-1::gfp) in control and mid-L4 stressed males at day 1 of adulthood (arrowheads indicate DVB neurites with no GFP signal). Bar, 10 μm. (E) Number of GFP puncta in DVB neurites in control and mid-L4 stressed males at day 1.