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. 2019 Nov 18;11(11):2810. doi: 10.3390/nu11112810

Figure 2.

Figure 2

Sdc4 deficiency leads to an increase in visceral adipocyte size and macrophage infiltration in female mice fed an HDF for 14 weeks. (A) Representative haematoxylin and eosin of gonadal WAT (gWAT) sections from mice fed an HFD for 14 weeks. Black arrows in top right panel depict cell infiltration. (B,C) Cumulative relative frequencies (CRF) distribution of adipocyte size from female (Panel B) and male (Panel C) mice (100 cells per animal; n = 3–4). (D) Gene expression levels were measured by qPCR using mRNA isolated from gWAT. Box and whiskers plots denote individual data points, separated by a line representing the group median. Each individual value is plotted as a dot superimposed on the boxplots (n = 5). Transcript levels of each target gene were normalized to Hprt, Actb, and Tbp. (E) Representative images of immunohistochemical staining for F4/80 protein. (F) Data represent means for n = 3–4 animals, with five not overlapping images taken per animal. Error bars represent standard errors. In panels D and F, * p < 0.05 and ** p < 0.01, compared to WT.