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. 2019 Dec 5;8:e50583. doi: 10.7554/eLife.50583
Genotyping primers
cam-1(gm122):
Forward: aacttacactctgaagcgccgtgc
Reverse: ccaacttcagatgatggatcg
egl-20(n585): wildtype PCR product can be digested with HpyCH4V:
Forward: cttacctctcaaatttgaacttattcttgc
Reverse: cctcattaccattcaactgatag
fmi-1(tm306):
Forward: gtgaaattacatgttaactgagg
Reverse: tcgcctacaagaagtaacttacatg
lin-44(n1792): wildtype PCR product can be digested with NcoI:
Forward: gtgcgaatcgtttgagatttcagccatg
Reverse: catctggttgttacacgcacaatcg
mig-1(e1787): mutant PCR product can be digested with BgIII:
Forward: acagcacaaaattcaaagccctc
Reverse: atttttgagccattcaaaaagaattttgaagatc
sax-7(nj48):
Forward: tgagatgaaagaaggaggagtgc
Reverse: cacacacaatggcgcacaag
lin-17(n671): wildtype PCR product can be digested with MfeI:
Forward: ccgcatttttcgtagatcacaccg
Reverse: actgttgtttacagtcaattgtcattcgggtcaatt
vang-1(ok1142):
Forward: accttgacaacgccagacag
Mutant reverse: gcggcaattaggcgatacctg
Wildtype reverse: cgagcgaagtttgagtgaatc
dsh-1(ok1445):
Forward: cttgagatagccctgcaagac
Reverse: gctccaccacttgctaagattg
mig-5(tm2639):
Forward: aagcattcgctcctcatcatc
Reverse: atctcgacgatgaaacgactc
nrt-bfp-lin-44(miz56):
Wildtype forward: ggaaatagtgtgtggatgag
Mutant forward: aaggaaccgtggacaaccatc
Reverse: ccactgatcgtcgggatctc
sdn-1(ok449):
Forward: agaagtgtctcgttcagtgg
Wildtype reverse: ctgcaaagccgacaacggtac
Mutant reverse: gagatgccggtcaggtgattac
cwn-1(ok546):
Forward: agctggctaggtcccagaag
Reverse: tcgaacgatcctcttcagtac