Fig. 8.

Seed storage proteins 2S albumin and 12S globulin show premature accumulation in the e2f mutant siliques and seeds. (A) Accumulation levels of 12S globulin in the single e2fa-2, e2fb-1 and in the double e2fa-2/e2fb-1 mutants at three silique and seed developmental stages (S1-S3) were compared with the control WT in immunoblot assay using a specific antibody. (B,C) SSPs were detected in the early developing siliques and seeds of e2fa-1, e2fa-2, e2fb-1 and e2fb-2 single, as well as e2fa-2/e2fb-1 double mutants by using anti-12S (B) or anti-2S (C) antibodies in immunoblot assays. The WT maturation-phase silique sample (S3) was used as positive control, and a quarter of the S1-S2 samples were analysed (5 µg). Arrowheads indicate precursors of 12S or 2S proteins. (D) The amount of globulin (12S) and albumin (2S) in post-mature seeds (S4) of WT, single and double activator e2f mutants were compared using specific anti-12S and anti-2S antibodies in immunoblot assays. Coomassie-stained proteins were used as loading controls.