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. 2019 Jul 17;20(21):2685–2710. doi: 10.1002/cbic.201900072

Figure 11.

Figure 11

Concept of CEST experiments. Normalized intensities of peaks observed at different saturation offsets are fitted and k EX, p ES and the chemical shift of the excited stated is extracted. This chemical shift information of the excited state allows for the hypothesis of the excited state structure, and mutants stabilizing this state (mimics) need to be designed to make it the observable state for confirmation. If the mimic was chosen successfully, its CEST profile will show only one dip, as exchange will be suppressed at the chemical shift position where before the excited state chemical shift was found. Fitting CEST data to an appropriate exchange model will yield qualitative analysis of chemical shift difference and extraction of exchange rate and populations. Reprinted with permission from ref. 42.