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Study design overview. C57BL/6L female mice were placed on either CD (n=10) or HFHS (n=16) for a total 8 weeks. At 4 weeks of diet challenge, (designated week 0 of treatment) mice were subcutaneously implanted with osmotic minipumps infusing saline or ADM22–52 (1.2 mg/kg wt/day). IPGTT performed at weeks as indicated. Animals were sacrificed after treatments and visceral adipose tissue (VAT) collected for explant culture.
