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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Endocrine. 2019 Apr 25;65(2):278–285. doi: 10.1007/s12020-019-01927-8

Figure 4.

Figure 4.

Serum insulin levels and mRNA of Insr and Glut4 by VAT in mice under CD or HFHS diets. (A) Serum insulin values were assessed using a Rat/Mouse insulin ELISA. qPCR was performed to determine the mRNA expression of (B) Insr and (C) Glut4 in VAT obtained from mice under HFHS or CD diets treated with or without ADM22–52. mRNA was normalized to the average of three housekeeping genes, β-actin, Gapdh and Hprt and data displayed as mean+/−SEM. Different letters indicate significant difference (p<0.05).