Fig. 2.

Similarities of GluN1-immunogold distribution in neuronal profiles contacted by other neuronal and glial structures in the PL-PFC of adult mice that received vehicle or Δ9-THC. GluN1 is seen within the cytoplasm (small arrows) and on plasma membranes (circles) of selective neuronal (dendritic and axonal) and perisynaptic glial profiles in the PL-PFC of adult mice that received vehicle (a, b) or ∆9-THC (c, d) during adolescence. In these images GluN1-immunogold particles are seen in dendritic spines (GluN1-s) receiving asymmetric synapses (curved arrows) from unlabeled terminals or GluN1-labeled terminals. GluN1 immunogold is also respectively localized together (Du-Te) or separate (GluN1-Te) from dense CB1R-immunoperoxidase labeling in axon terminals forming symmetric synapses (block arrows) with GluN1-labeled somatodendritic profiles (GluN1 Soma) and dendrites (GluN1-De). e Dual labeled terminals (Du-te) contain sparse gold GluN1 and CB1-immunoperoxidase labeling (chevron). In f, unlabeled axon terminals form asymmetric synapses (curved arrows) with a dendritic spine that is contacted by the dual labeled terminal (upper left) and an unlabeled dendritic spine (Un-s) opposed to the GluN1-De. tv = tubulovesicles (tv), mit = mitochondrion, U-Te = unlabeled terminal; Un-S = unlabeled spine; scale bar = 500 nm. A GluN1-immunogold labeled dendrite (GluN1-De) is opposed to a GluN1-labeled terminal (GluN1-Te) and to a perisynaptic glial process (double dashed line) in g and h, respectively