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. 2019 Nov 22;8:e49455. doi: 10.7554/eLife.49455

Figure 3. Translation is reduced globally during oocyte aging.

Figure 3.

(A) Relative read depths from replicate ribosome footprinting and mRNA-sequencing experiments of Hsp26, vtd, and me31B from 2-, 8-, and 12-day-old oocytes. Data were normalized to spike-in controls. Relative translational efficiency (right panel) falls 2.5–5 fold between day 2 and day 12. (B) Read depths and translational efficiency values are shown as in (A) for genes preferentially translated in embryos CycB, CycA, and bora from 2-day oocytes, 12-day oocytes, and 0–2-hr embryos from non-aged oocytes. (C) Heat maps showing reduced translation, but similar mRNA levels, of genes of various GO categories in 12-day oocytes as compared to 2-day oocytes. Gene classes of interest are indicated on the left, along with specific genes on the right; see Flybase for information on each gene (http://flybase.org). Error bars in (A) and (B) denote SD.