Figure 6. Deletion of CB2Rs does not worsen flurothyl-induced seizures in Scn1a^RH/+ mutant mice.

A. Male Cnr2^−/−/RH (teal stripe) and Cnr2^+/−/RH (gray stripe) mutants exhibit decreased latency to the MJ when compared to WT (black) (^##P < 0.01, ^###P < 0.001). Cnr2^+/−/RH mutants (gray stripe) also show decreased latency to the MJ when compared to RH (black stripe) (*P < 0.05) and Cnr2^+/− (gray) (**P < 0.001). B. When compared to WT, RH (black stripe) (^#P < 0.05), Cnr2^+/−/RH (gray stripe) (^###P < 0.001), Cnr2^−/− (teal) (^##P < 0.01 ), and Cnr2^−/−/RH (teal stripe) male mutants (^##P <0.01) exhibit significantly decreased latencies to GTCS. Cnr2^+/−/RH mutants (gray stripe) exhibit significantly decreased latency to the GTCS compared to Cnr2^+/− mice (gray) (*P < 0.05). C. Cnr2^−/− /RH female mice (teal stripe) show significantly decreased latency to the MJ when compared to WT (black) (^##P < 0.01), RH (black stripe) (*P < 0.05), Cnr2^+/− (gray) (***P < 0.001), and Cnr2^−/−(teal) (*P < 0.05) mice. D. Cnr2^−/− /RH female mice (teal stripe) exhibit decreased latency to the GTCS when compared to WT (^#P < 0.05) and Cnr2^+/− mutant mice (gray) (**P < 0.01). 2-way ANOVA with Tukey’s post hoc comparison, n=8–12/group. Symbols above individual bars indicate significance from WT. Error bars represent mean +/− SEM