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The purity and adipogenic differentiation of goat muscle satellite cells. (A) Immunofluorescence analysis of Pax7 in goat muscle satellite cells. Cells were stained with PAX7 antibody and nuclei were stained with DAPI. Scale bars: 100 μm. (B) Relative expressions of adipogenic-regulated genes (FABP4, FAS and PPARγ) during cell differentiation at 0, 4 and 7 days by qPCR. Error bars represent SEM of three separate experiments. ** p < 0.01 relative to 0 d level.
