Table 2.
Dyes and antibodies used for the stainings.
| Dye/Antibody | Type/Species | Company | Cat. Nr. | Dilution |
|---|---|---|---|---|
| 4′,6-Diamidin-2-phenylindol (DAPI) | - | Sigma Aldrich | 1:15,000 | |
| O1 | Monoclonal/Mouse | Kindly provided by Prof. M. Schwab, Zürich, CH | D9542-10 mg | 1:250 |
| Platelet derived growth factor alpha (PDGFRa) | Polyclonal/Rabbit | Kindly provided by Prof. William B. Stallcup, La Jolla, CA, US | 1:4000 | |
| Myelin basic protein (MBP) | Monoclonal/Rat | Merck Millipore | 1:250 | |
| Myelin oligodendrocyte glycoprotein (MOG) | Monoclonal/Mouse | Kindly provided by Prof. R. Reynolds, London, UK | MAB386 | 1:250 |
| Oligodendrocyte linear factor 2 (OLIG2) | Polyclonal/Rabbit | Merck Millipore | Clone Z12 | 1:2000 |
| Glial fibrillary acidic protein (GFAP) | Polyclonal/Chicken | Aves Labs | AB9610 | 1:2000 |
| dk–a–m–488 | Monoclonal/Donkey | Jackson ImmunoResearch | AB_2313547 | 1:700 |
| dk–a–rb–594 | Monoclonal/Donkey | Jackson ImmunoResearch | 1:700 | |
| dk–a–rt–647 | Monoclonal/Donkey | Jackson ImmunoResearch | 715-545-140 | 1:700 |
| dk–a–ck–647 | Monoclonal/Donkey | Jackson ImmunoResearch | 711-585-152 | 1:700 |
| 712-605-150 | ||||
| 703-605-155 |