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. 2019 Oct 31;8(11):1360. doi: 10.3390/cells8111360

Figure 1.

Figure 1

HOXA9 represses cell proliferation while promotes apoptosis in cSCC cells. (a) HOXA9 protein expression was detected after knockdown of HOXA9 by siRNAs in cSCC cells. Measurements of cell proliferation by CCK-8 assay (n = 3) (b) and apoptosis assay by Annexin V/PI double staining (n = 3) (c) were performed in cSCC cells treated with siRNAs targeting HOXA9. (d) HOXA9 protein expression was detected by western blot after overexpression of HOXA9 in cSCC cells. Measurements of cell proliferation by CCK-8 assay (n = 3) (e) and apoptosis assay by Annexin V/PI double staining (n = 3) (f) were performed in cSCC cells overexpressing HOXA9. (g) The autophagy in cSCC cells following HOXA9 knockdown was evaluated by LC3 staining. * p < 0.05, ** p < 0.01, *** p < 0.001.