Figure 2.

Cytokine profile measured in the culture of splenocytes isolated from T. spiralis-infected mice: On days 6, 15, 30, and 60 after infection with T. spiralis, four infected mice at each time point were sacrificed and splenocytes were isolated. The culture supernatants were collected after being incubated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 72 h, and the concentrations of IL-4, IL-17A, IFN-γ, IL-6, TGF-β, IL-21, IL-10, and IL-12/23p₄₀ were measured by ELISA. Data are representative of three independent experiments, expressed as means ± SDs, * p < 0.05; ** p < 0.01; *** p < 0.001, compared to the uninfected group.