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. 2019 Nov 7;8(11):1404. doi: 10.3390/cells8111404

Figure 4.

Figure 4

mRNA expression profile for Treg-related marker proteins in mice immunized with AES and MES: BALB/c mice were immunized with AES, MES, or PBS. (A) The CD4+CD25+ and CD4+CD25 T cells were acquired by FACS, and the mRNA for Foxp3+, TGF-β, and IL-10 was measured by RT-PCR. (B) CD4+Foxp3+ T cells were isolated by FACS for mRNA analysis of Foxp3, IL-10, and TGF-β. Results are presented as means ± SDs from three independent experiments normalized to the level of HPRT 1. ** p < 0.01; *** p < 0.001, compared to the PBS control.