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. 2019 Nov 7;8(11):1404. doi: 10.3390/cells8111404

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TsES-primed BMDCs upregulated Foxp3⁺ CD4⁺CD25⁺ T cells populations and Treg cytokines in naive T cells. T cells were isolated from naive BALB/c mice splenocytes and co-cultured with AES-, MES-, LPS-, BSA-, or PBS-treated BMDCs in the presence of anti-CD3/anti-CD28 for 48 h. (A) The cells were sorted with CD3, CD4, CD25, and Foxp3 by FACS. (B) Percentage of CD25⁺Foxp3⁺ cells in a CD4⁺ T cell population is shown. (C) Cytokines in co-cultured supernatants were detected by ELISA. Data are shown as the means ± SDs for three independent experiments. Results represent one of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001, compared to PBS or BSA control.