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. 2019 Nov 4;8(11):1388. doi: 10.3390/cells8111388

Figure 5.

Figure 5

Resting T cells constitutive internalization of the TCR-CD3 complex is impaired by Cdc42-Q61L expression. (A) Remaining surface TCR-CD3 detected by an antibody against TCRβ at the indicated timepoints in cells expressing GFP-Cdc42-WT, GFP-Cdc42-Q61L, or GFP-Cdc42-T17N. (B) Total surface TCR-CD3 in resting cells expressing the same constructs as in (A) over time. (C) Percentage of TCR internalized from cell surface in respect to the amount detected at t = 0 in activated cells (anti CD3ε, data from Figure 4B) or resting cells (TCRβ) at the indicated time points. (D) Extent of impairment in TCR internalization upon expression of GFP-Cdc42-Q61L (in %) in respect to expression of GFP-Cdc42-WT in activated or resting cells. Each data point represents the mean of an individual experiment. Small horizontal lines indicate mean (±SEM), * p  <  0.05, ** p  <  0.01, *** p  <  0.001, unpaired, two-tailed student’s t-test.