Figure 3.

The stimulating activity of Wnt3a-conditioned media (Wnt3a CM) was suppressed after GO-treatment in reporter gene assays. HEK-293 (A) or Neuro-2a (B) cells were co-transfected with Topflash (TOP) or Fopflash (FOP) plasmids together with the Renilla plasmid, which was used as an internal control for transfection efficiency. Prior to control CM and Wnt3a CM stimulation, media were pre-incubated with indicated concentrations of GO for 3 h at 37 °C, then added to the transfected cells for additional 18 h. Luciferase reporter activity was measured. The graphs show mean values ±SD of n = 3 independent experiments, each measured in duplicate. Circles represent individual experiments. Differences to non-glycated Wnt3a CM were analyzed by one-way ANOVA analysis. * p < 0.05, ** p < 0.01.