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. 2019 Nov 5;8(11):1394. doi: 10.3390/cells8111394

Figure 5.

Figure 5

Influence of autophagy-related ULK-1 and IRGM proteins expression on bacterial clearance within MDM from blood donors. (A) MDM were transfected with either control siRNA, ULK-1 or IRGM siRNA. ULK-1 and IRGM proteins level was analyzed by western blotting. (B) Number of intra-macrophagic AIEC LF82 bacteria at 6 h post-infection within MDM after control, ULK-1 or IRGM siRNA transfection. (C) Western blot and quantification of P62 level in MDM infected or not by AIEC LF82 at 1 h and 6 h post-infection. (D) Western blot and quantification of P62 level in MDM, after siCTL, siULK-1 or siIRGM transfection after AIEC LF82 infection or not at 6 h post-infection. All MDM were infected by AIEC LF82 at MOI 100. For the western blots, quantification is expressed as amount relative to GAPDH and to uninfected MDM or control siRNA. Data represent results from at least three independent experiments. (mean ± s.e.m., * p < 0.05).