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Bar graph indicating the number of neuropeptides detected in brain tissue extract samples when analyzing a direct spot, 60 s CE fractions, 30 s CE fractions, and a continuous CE trace using MSI. Error bars represent the standard deviation, and n corresponds to the number of replicates performed. An ANOVA test was performed (p-value 1.0×10−4) followed by a Dunnett’s post-hoc test comparing each method to the continuous trace. Asterisks indicate statistical significance at α < 0.05.
