Figure 1.

Experimental design in this study. Two crude extract pools were prepared from HT-29 and AsPc-1 cell cultures. The two samples were dispensed and distributed to 12 participating laboratories. In each laboratory, the peak was identified for each metabolite and their relative signal intensity value (HT-29/AsPc-1) was measured according to their sample pretreatment, separation, detection, and data processing methods. Data were compiled and analyzed using statistical tests.