The article entitled, “Low-Intensity Pulsed Ultrasound Stimulation Enhances Heat-Shock Protein 90 and Mineralized Nodule Formation in Mouse Calvaria-Derived Osteoblasts” by Miyasaka et al. (DOI: 10.1089/ten.tea.2015.0234), was published in Tissue Engineering, Part A, Volume 21, Numbers 23 and 24, pages 2829–2839. The authors have discovered errors in Figure 1 and Table 1.
Table 1.
Primer Pairs Used for Real-Time PCR
| Gene | Forward and reverse primers | GenBank™accession number | Fragment length(bp) |
|---|---|---|---|
| GAPDH | ACCCAGAAGACTGTGGATGG | NM_001289726 | 186 |
| CACATTGGGGGTAGGAACAC | |||
| HSP27 | CCCAGTGAATCCCCTGTCTA | NM_001164708.1 | 179 |
| CCCCCAGGTTTTGGTTTATT | |||
| HSP70 | TGCTGATCCAGGTGTACGAG | NM_010478.2 | 204 |
| CGTTGGTGATGGTGATCTTG | |||
| HSP90 | GGCATCGATGAAGATGAGGT | NM_008302.3 | 196 |
| ACATGAGCAGAGAGCCAGGT | |||
| Smad1 | TCTTTCTGAAGTGGGCTTTC | NM_008539.3 | 185 |
| CAGCCAGCATACAGTTTCAG | |||
| Smad5 | TGTGCTCCTTGTGCAGATAA | NM_008541.3 | 221 |
| TGGTGCTCTACGAGACCTTC | |||
| OSX | CCCACCTAACAGGAGGATTT | NM_130458.3 | 200 |
| CACTGGAATGGAGTGAAACC | |||
| BMP2 | AGCAAGGACGTCGTGGTGCC | NM_007553.3 | 202 |
| ATTATTTCGGTGCTGGAAACTACT |
HSP, heat shock protein.
In Figure 1A(1) the words “Treatment 2” should have been deleted. In Table 1 the “Colla1” primer set should have been replaced by the “BMP2” primer set. The corrected Figure 1 and Table 1 appear below.
In the text the only change is to the first paragraph in the “Results” section. The parenthetical citation at the end of the paragraph has been changed from (Fig.2C) to (data not shown). The paragraph now ends with the sentence,
“Intense staining was obvious in control osteoblasts, although this group contained the fewest cells (data not shown).”
The remaining data and the conclusion are unchanged.
The authors apologize for these errors.